Product Number: DN32-HS

Shipping and Storage

Stored at -20℃, with a shelf life of 1 year.

Description

Hot Start dNTP Mixture is a dNTP product that has undergone special chemical modifications. Under normal circumstances, Hot Start dNTP Mixture cannot participate in the generation of phosphodiester bonds. Only after heat shock, Hot Start dNTP Mixture will become a regular dNTP and participate in PCR amplification. This product can significantly reduce non-specific amplification caused by non-specific annealing or primer dimers.

Protocol

1. This product is sensitive to temperature and should be avoided from repeated freezing and thawing. It is recommended to pack it in advance before use and thaw it on ice during use.
2. This product begins to show a thermal activation trend at 50-60℃, and when the temperature reaches 95℃, heating for 30-60 seconds can complete the thermal activation.

Note

1. Hot Start dNTP Mixture can be stored at 4℃ for up to 15 days, but it is not recommended to store at room temperature.
2. When using cDNA as a template, it is recommended to use commercially available purification kits to remove unadulterated nucleotides. If the experimental protocol requires the use of unpurified cDNA products, the reaction volume of cDNA synthesized products in the PCR system should not exceed 1/10.
3. In the multiplex reaction of amplifying four or more targets, it is recommended to try adding KCl with a final concentration of up to 100mM to improve the results. The KCl content in the PCR buffer should be considered during the optimization process.

Description

dNTP Set consits of 100 mM aqueous solutions at pH 7.0 of each of dATP, dGTP, dCTP and dTTP, each in a separate vial.

Because the nucleotides are provided in separate solutions, dNTP sets offer maximum flexibility in preparation of reaction mixes for different applications.

Quality control

Functionally tested in PCR with Taq and Pfu DNA Polymerases.
Greater than 99% purity of each component confirmed by HPLC.
Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities.

Applications

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling.
IVD use available!
For IVD customer we can offer bulk package!

Storage

Long Term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C

Standard package

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available

Delivery Time

2 working days

Order

Description

dNTP Mix 2.5mM each is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 2.5mM in water.
So the total concentration of nucleotides therefore is 10mM (pH 7.4). dNTP mixes are ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications.

Quality Control

Functionally tested in PCR with Taq and Pfu DNA Polymerases.
Greater than 99% purity of each component confirmed by HPLC.
Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities.

Application

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension and DNA labeling.
IVD use available!
For IVD customer we can offer bulk package!

Storage

Long Term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C

Standard package

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available

Delivery Time

2 working days

Order

Description

dNTP Mix 10mM each is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10mM in water;
So the total concentration of nucleotides therefore is 40mM (pH 7.4). dNTP mixes are ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications.

Quality Control

Functionally tested in PCR with Taq and Pfu DNA Polymerases.
Greater than 99% purity of each component confirmed by HPLC.
Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities.

Application

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension and DNA labeling.
IVD use available!
For IVD customer we can offer bulk package!

Storage

Long Term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C

Standard package

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available

Delivery Time

2 working days

Order

Description

dNTP mix 25mM each is a premixed solution by 100mM of dATP, dGTP, dCTP and dTTP.
The concentration of each component in the solution is 25mM.

Quality Control

Purity>99% (HPLC)

Application

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension and DNA labeling.
IVD use available!
For IVD customer we can offer bulk package!

Storage

Long Term (Infrequent use; 1-2 times per month): -70°C
Daily/Weekly use: -20°C

Standard package

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available

Delivery Time

2 working days

Order

Description

This dNTP/dUTP mix are manufactured by MEBEP Bio Science, one of a few primary manufacturers in the industry.

The dNTP/dUTP Mix 10mM each is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dUTP, each at 10mM in water.
So the total concentration of nucleotide therefore is 40mM. For direct use in PCR with Taq DNA Polymerase, reverse transcription and other applications.

Quality Control

Functionally tested in PCR with Taq DNA Polymerase.
Greater than 99% purity of each component confirmed by HPLC.
Free of human and E. coli DNA.
Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.

Applications

For use in all molecular biology applications including PCR, real-time PCR & RT-PCR with Taq DNA Polymerase, cDNA synthesis, MDA and DNA sequencing.

Note

dNTP/dUTP mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

Storage

Store at -20°C.

Standard package

1ml/vial
10ml/vial
25ml/bottle
50ml/bottle
Other package sizes are available, OEM and Bulk both are available

Delivery Time

2 working days

Order