Product Number: DI05

Shipping and Storage

 -20℃，Valid for 12 months.

Components

Description

DNase I is a non-specific nuclease cleavage enzyme, mostly derived from recombinant E. coli strains, containing MBP fusion clones of bovine pancreatic DNase I. DNase I can be used to degrade single or double stranded DNA, based on the principle that DNase I hydrolyzes phosphate diester bonds to produce single or oligonucleotides with 5 ‘- phosphate groups and 3’ – OH.

Both Mg²⁺ and Mn²⁺ can activate the activity of DNase I, and the concentration of Ca²⁺ directly affects the activity of the enzyme. When Mg²⁺ is present, it can randomly generate incisions on each single strand of double stranded DNA; In the presence of Mn²⁺, double stranded DNA can be broken, resulting in DNA fragmentation.

DNase I solution (1mg/ml) is composed of DNase I, enzyme protection solution, preservatives, etc., with a concentration of 1mg/ml, used for single stranded DNA, double stranded DNA, chromatin, RNA: DNA hybrid strands. It is commonly used for the preparation of DNA free RNA, reverse transcription, and in vitro transcription experiments.

Unit definition

1 unit refers to the amount of enzyme required to completely degrade 1μg of pBR322 DNA in a 50μl reaction system at 37℃ for 10 minutes.

This product is a GMP grade recombinant deoxyribonuclease I (DNase I), which can randomly decompose single or double stranded DNA to produce 5′- p-terminal oligonucleotides. DNase I can cut double stranded DNA at will under Mg2+; Under Mn2+ condition, DNase I can cut DNA double strand at the same site to form flat end or stick end with 1-2 nucleotides protruding.

This product is a large-scale expression of recombinant DNase I in E. coli, with a molecular weight of about 39kD, which conforms to GMP production and quality management specifications. All raw and auxiliary materials are traceable.

Specification

• Heavy metal: ≤ 10ppm
• No virus, mycoplasma and other common pathogens
• Internal toxicity control: ≤ 10 EU / mg
• Volume activity(containing cCMP / RNase A): ≥ 40 Ku / ml
• Host protein residue: ≤ 50 ppm
• No RNase activity, cleavage activity
• Host DNA residue: ≤ 100 pg / mg
• There was no nonspecific nuclease activity

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