Product Number: DB005

Shipping and Storage

-20℃.

Components

Description

This product is a Cre recombinase obtained by expressing the plasmid encoding the bacteriophage P1 Cre protein in Escherichia coli and purifying and isolating it multiple times. This enzyme does not require energy cofactors, and Cre – mediated recombination quickly reaches equilibrium between the substrate and the reaction product.

Cre recombinase is a type I topoisomerase of bacteriophage P1, catalyzing site-specific recombination of DNA between loxP sites. The loxP recognition element is a 34bp sequence with two 13bp inverted repeat sequences at both ends and an 8bp spacer in the middle for directional purposes. The recombinant product varies depending on the position and relative direction of the loxP site, and two DNA containing a single loxP site will be fused. The DNA between two forward repeating loxP sites will be cleaved in a circular form, while the DNA sequence between two reverse loxP sites will be flipped.

Application

1. DNA cleavage between two loxP sites;
2. Fusion of DNA molecules containing loxP sites;
3. Flipping of DNA sequences between loxP sites.