Product Number: BS03

Shipping and Storage

Store at -20°C.

Components

Description

WellStart III BST DNA polymerase is a hybrid enzyme containing WarmStafi Bst DNA polymerase and a thermostable reverse transcriptase. WarmStafi Bst III features modifications over Bst 3.0, eliminating nonspecific amplification during room-temperature reaction setup and eliminating the need for a separate activation step, thereby enhancing reaction specificity. The thermostable reverse transcriptase is a genetically engineered novel enzyme with significantly improved cDNA synthesis speed and thermal stability, capable of tolerating reaction temperatures up to 60°C, making it suitable for reverse transcription reactions with RNA templates possessing complex secondary structures. WellStart III BST DNA polymerase can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT-LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80 ℃ for 5 minutes to inactivate.

Product Number: BS07

Shipping and Storage

Transportation under 0°C and storage at -25~ -15°C.

Component

Description

Bst DNA polymerase V2 is derived from Bacillus stearothermophilus DNA Polymerase I, which has 5´→ 3´ DNA polymerase activity and strong chain replacement activity, but no 5´→3´ exonuclease activity. Bst DNA Polymerase V2 is ideally suitable for strand-displacement, isothermal amplification LAMP (Loop mediated isothermal amplification) and rapid sequencing. Well Start Bst DNA polymerase,large fragment is a hot-start version based on Bst DNA polymerase V2 obtained by reversible modification technology, which can inhibit DNA polymerase activity at room temperature, so the reaction system can be operated and formulated at room temperature to prevent non-specific amplification and improve reaction efficiency, and this version can be lyophilized. In addition, its activity is released at high temperatures, so there is no need for a separate activation step.

Application

1. LAMP isothermal amplification;
2. DNA strand single displacement reaction;
3. High GC gene sequencing;
4. DNA sequencing of nanogram level.

Unit Definition

One unit is defined as the amount of enzyme that incorporate 25nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Heat Inactivation

80℃, 20 min.

Product Number: BSM03W

Shipping and Storage

-20°C.

Components

Description

WellStart Bst 3.0 Enzyme Mix is a mixed enzyme that contains WellStart Bst DNA polymerase and high-temperature resistant reverse transcriptase.WellStart Bst 3.0 Enzyme Mix adds modifications on top of Bst 3.0, eliminating non-specific amplification generated during reaction establishment at room temperature and eliminating the need for a separate activation step, which can improve the specificity of the reaction.High temperature resistant reverse transcriptase is a new enzyme modified by genetic engineering, with fast cDNA synthesis speed and significantly improved thermal stability. It can withstand reaction temperatures up to 60℃ and is suitable for reverse transcription reactions of RNA templates with complex secondary structures.WellStart Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80℃ for 5 minutes before inactivation.

Product Number: BSM03

Shipping and Storage

-20°C.

Components

Description

Bst 3.0 Enzyme Mix is a mixed enzyme containing Bst DNA polymerase and high-temperature resistant reverse transcriptase. Bst DNA polymerase is a recombinant enzyme expressed and purified through E. coli, which undergoes partial point mutations on the basis of the original sequence. It has stronger 5’→3′ DNA polymerase activity, strand displacement activity, reverse transcriptase activity, and no 5’→3′ exonuclease activity.High temperature resistant reverse transcriptase is a new enzyme modified by genetic engineering, with fast cDNA synthesis speed and significantly improved thermal stability. It can withstand reaction temperatures up to 60℃ and is suitable for reverse transcription reactions of RNA templates with complex secondary structures.Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.

Unit definition

This product is suitable for various isothermal amplification reactions such as RT LAMP, LAMP, RCA, CPA, etc.

Heat Inactivation

Incubate at 80℃ for 5 minutes before inactivation.

Manual

Product Number: BS02

Shipping and Storage

-20°C.

Components

Description

WellStart II BST DNA polymerase is a recombinant enzyme expressed and purified by Escherichia coli. Its gene is derived from Bacillus stearothermophilus and has undergone partial point mutations based on the original sequence.This protein has stronger 5’→ 3′ DNA polymerase activity, chain displacement activity, reverse transcriptase activity, and no 5’→ 3′ exonuclease activity.Applied to DNA/RNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), etc.

Unit definition

The amount of enzyme required to add 10nmol of deoxynucleotides to acidic insoluble substances within 30 minutes at 65℃ is defined as 1 active unit (U).

Heat Inactivation

Incubate at 80°C for 5 minutes before inactivation.

Quality Control

After multiple column purification, the purity was detected by SDS-PAGE to be greater than 98%; No exogenous nuclease activity was detected.

Test

No activity at room temperature. Will be activated at 60°C.

Product Number: BS01

Storage Conditions

-20°C.

Components

Description

Bst DNA Polymerase (Bst), a Large Fragment, is a purified recombinant enzyme expressed by Escherichia coli. Its gene was derived from Bacillus stearothermophilus and some point mutations were carried out on the basis of the original sequence.The protein has 5’→3′ DNA polymerase activity, but no 5’→3′ and 3’→5′ exonuclease activity, and has strong chain replacement activity. It should be used for DNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), library sequencing, etc.

Unit definition

The amount of enzyme required to incorporate 10nmol of deoxynucleotides into an acid insoluble substance at 65°C for 30 min is defined as 1 activity unit (U).Thermal inactivation: inactivation after incubation at 80°C for 20min.

Quality Control

After several column purification, its purity was more than 98% detected by SDSPAGE. No exogenous nuclease activity and no host residual DNA were detected.

Product Number: BS06

Shipping and Storage

Store at -30 ~ -15 ℃ and transport at ≤ 0 ℃.

Components

Note:1) Store in 10 mM Tris HCl pH 7.5, 50 mM KCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol.

2)Self provided material: Reagent:dNTP Mix，FIP/BIP Primers，F3/B3 Primers，LoopF/LoopB Primers，Nuclease-free ddH2O。

Instrument: qPCR instrument, PCR instrument or water bath.

Description

Bst 2.0 Pro DNA Polymerase is a directed modification of a large fragment of Bacillus stearothermophilus DNA polymerase, which exhibits 5 ‘→ 3’ DNA polymerase activity and strong strand displacement activity, but lacks 5 ‘→ 3’ exonuclease activity. Bst 2.0 Pro DNA Polymerase can be used for isothermal amplification reactions, such as LAMP (Loop Mediated Isothermic Amplification), HDA (Helicate Dependent Amplification), RCA (Rolling Circle Amplification), etc. Compared with Bst 2.0 DNA Polymerase, Bst 2.0 Pro DNA Polymerase, combined with a new generation of hot start technology, can inhibit polymerase activity at temperatures below 50 ℃ and rapidly release enzyme activity above 50 ℃. It has higher amplification speed, specificity, salt resistance, and thermal stability, and supports the preparation of reaction systems at room temperature.

Source

Derived from Bacillus stearothermophilus.

Application

This product is suitable for various isothermal amplification reactions such as LAMP, HDA, RCA, etc.

Unit definition

The amount of enzyme required to add 10 nmol of dNTP to acid insoluble precipitate within 30 minutes at 65 ℃ is defined as one active unit (U).

Note

1. This product is for scientific research purposes only and shall not be used for clinical medical diagnosis or other unreasonable purposes.
2. This product is not suitable for PCR reactions.
3. The operating temperature of this product does not exceed 70 ℃.

Product Number: BS05

Shipping and Storage

-20℃.

Components

*The reaction buffer contains 40mM Mg

Description

BST 2.0 DNA Polymerase is a homolog of Bacillus stearothermophilus DNA polymerase (BST DNA Polymerase, Large Fragment), derived from E.coli strain. Obtained through multiple purification and isolation after expression in Escherichia coli using gene recombination technology. This enzyme has 5′→ 3′ DNA polymerase activity, but lacks 5′→ 3′ exonuclease activity. Compared with wild-type BST DNA polymerase large fragments, BST 2.0 DNA polymerase exhibits higher amplification speed, yield, and sensitivity.

Features

1. Compared with large fragments of BST DNA polymerase, the amplification speed and sensitivity have been significantly improved.
2. Optimization was carried out for loop mediated isothermal DNA amplification (LAMP).
3. Strong chain displacement activity.

Application

1. DNA sequencing rich in GC sequences;
2. Rapid sequencing of trace (nanogram) DNA templates;
3. Random primer DNA labeling;
4. Double stranded DNA 5 ‘protruding end patch labeling method;
5. It can be used for isothermal DNA amplification, such as loop mediated isothermal amplification (LAMP), whole genome amplification (WGA), helicase isothermal gene amplification (HDA), etc.

Unit definition

The amount of enzyme required to add 25nmol of dNTPs to acid insoluble precipitate within 30 minutes at 65 ℃ is defined as one active unit.

Product Number: BS04

Shipping and Storage

-20°C.

Components

Description

Bsu DNA polymerase is a full-length protein of DNA polymerase A from Bacillus subtilis, which has a 5 ‘→ 3’ exonuclease domain. However, this polymerase naturally lacks 3 ‘→ 5’ exonuclease activity.

Our company’s Bsu DNA polymerase is a recombinant protein expressed and purified through multiple steps.

Concentration

5U/μl

Features

1. Thermal deactivation: 75℃, 20min
2. Has certain incision translation activity

Application

1. RPA isothermal nucleic acid amplification reaction
2. Random primer labeling
3. Synthesis of the second strand of cDNA
4. Adding Tail to a Single dA

Unit definition

One unit is defined as the amount of enzyme that blends 10 nmol dNTP into an acid insoluble substance within 30 minutes at 37°C

Activity determination conditions

10×Reaction Buffer,37℃。

Storage buffer

50 mM Tris-HCl,50 mM KCl,1 mM DTT,0.1mM EDTA,50% Glycerol,pH 7.5,25°C。

Quality control

Relevant tests have shown that there is no contamination of exogenous endonuclease, exonuclease, or RNase. PCR method for detecting residual DNA without host.