Product Number: BSM03W

Shipping and Storage

-20°C.

Components

Description

WellStart Bst 3.0 Enzyme Mix is a mixed enzyme that contains WellStart Bst DNA polymerase and high-temperature resistant reverse transcriptase.WellStart Bst 3.0 Enzyme Mix adds modifications on top of Bst 3.0, eliminating non-specific amplification generated during reaction establishment at room temperature and eliminating the need for a separate activation step, which can improve the specificity of the reaction.High temperature resistant reverse transcriptase is a new enzyme modified by genetic engineering, with fast cDNA synthesis speed and significantly improved thermal stability. It can withstand reaction temperatures up to 60℃ and is suitable for reverse transcription reactions of RNA templates with complex secondary structures.WellStart Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80℃ for 5 minutes before inactivation.

Product Number: BSM03

Shipping and Storage

-20°C.

Components

Description

Bst 3.0 Enzyme Mix is a mixed enzyme containing Bst DNA polymerase and high-temperature resistant reverse transcriptase. Bst DNA polymerase is a recombinant enzyme expressed and purified through E. coli, which undergoes partial point mutations on the basis of the original sequence. It has stronger 5’→3′ DNA polymerase activity, strand displacement activity, reverse transcriptase activity, and no 5’→3′ exonuclease activity.High temperature resistant reverse transcriptase is a new enzyme modified by genetic engineering, with fast cDNA synthesis speed and significantly improved thermal stability. It can withstand reaction temperatures up to 60℃ and is suitable for reverse transcription reactions of RNA templates with complex secondary structures.Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.

Unit definition

This product is suitable for various isothermal amplification reactions such as RT LAMP, LAMP, RCA, CPA, etc.

Heat Inactivation

Incubate at 80℃ for 5 minutes before inactivation.

Manual

Product Number: BS02

Shipping and Storage

-20°C.

Components

Description

WellStart II BST DNA polymerase is a recombinant enzyme expressed and purified by Escherichia coli. Its gene is derived from Bacillus stearothermophilus and has undergone partial point mutations based on the original sequence.This protein has stronger 5’→ 3′ DNA polymerase activity, chain displacement activity, reverse transcriptase activity, and no 5’→ 3′ exonuclease activity.Applied to DNA/RNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), etc.

Unit definition

The amount of enzyme required to add 10nmol of deoxynucleotides to acidic insoluble substances within 30 minutes at 65℃ is defined as 1 active unit (U).

Heat Inactivation

Incubate at 80°C for 5 minutes before inactivation.

Quality Control

After multiple column purification, the purity was detected by SDS-PAGE to be greater than 98%; No exogenous nuclease activity was detected.

Test

No activity at room temperature. Will be activated at 60°C.

Product Number: BS01

Storage Conditions

-20°C.

Components

Description

Bst DNA Polymerase (Bst), a Large Fragment, is a purified recombinant enzyme expressed by Escherichia coli. Its gene was derived from Bacillus stearothermophilus and some point mutations were carried out on the basis of the original sequence.The protein has 5’→3′ DNA polymerase activity, but no 5’→3′ and 3’→5′ exonuclease activity, and has strong chain replacement activity. It should be used for DNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), library sequencing, etc.

Unit definition

The amount of enzyme required to incorporate 10nmol of deoxynucleotides into an acid insoluble substance at 65°C for 30 min is defined as 1 activity unit (U).Thermal inactivation: inactivation after incubation at 80°C for 20min.

Quality Control

After several column purification, its purity was more than 98% detected by SDSPAGE. No exogenous nuclease activity and no host residual DNA were detected.