dNTP

Biotin-11-dUTP 1mM Sodium solution

Description

Biotin-11-dUTP is enzymatically incorporated into DNA, cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA, cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose, magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine.

Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2′-deoxyuridine-5′-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.

Biotin-11-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptase, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.

Applications

Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension

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