Product Number: TR9810

Shipping and Storage

This product is transported at low temperature and stored at -20℃, with a validity period of 1 year to avoid repeated freezing and thawing. If it cannot be used all at once, 0.1ml can be divided into small portions for 0.2cm electric rotary cups, and 0.25ml can be divided into small portions for 0.4cm electric rotary cups.

Description

EZ Transfer electrotransfection reagent (electrotransfection buffer) is used to efficiently and low toxicity transfect nucleic acids (DNA, siRNA, etc.) into difficult to transfect cells. EZ Transfer is compatible with various conventional electrometers, including Lonza Amaxa®Nucleofector®,Bio-Rad®Gene Pulser and Harvard-BTX® electroporators. EZ Transfer can be used for exponential decay and square wave form electroporation experiments.

Important optimization tips

1 Lonza (Amaxa) cell nuclear power transfer instrument

When using the EZ Transfer transfection reagent with the Lonza (Amaxa) cell nuclear transfection instrument, please follow the operating guidelines and optimization plan of the Lonza (Amaxa) cell nuclear transfection instrument, just replace the electroporation mixture with EZ Transfer.

2 Cell density during electroporation

Determine the optimal cell density for each cell type to maximize the efficiency of electroporation. In the final system containing electroporation reagents, the cell density during electroporation is generally within the range of 1-10×10⁶ cells/ml. For suspended cells, it is best to have a high cell density close to 10×10⁶ cells/ml. For adherent cells, the recommended cell density is 1-5×10⁶ cells/ml. You can refer to Tables 1 and 2 to understand the cell density at which EZ Transfer reagents start.

3 Nucleic acid purity and concentration

• 3.1 Do not use DNA purified solely by ethanol precipitation method. The residual salt in the ethanol precipitation method can cause a change in current and have a negative impact on electroporation.
• The recommended concentration range for DNA extraction is 1-5 mg/ml, dissolved in pure water or TE. The use of high concentration DNA may lead to uneven mixing with cells. A lower nucleic acid concentration may dilute the electroporation mixture.
• 3.2 SiRNA uses high-purity and sterile siRNA.Determine the optimal siRNA concentration for electrotransfection. It is recommended to conduct gradient experiments with different concentrations of siRNA within the final concentration range of 250-750nM.

4 Pulse condition optimization

The EZ Transfer electrotransfection reagent can be used for both exponential decay electroporation experiments and square wave electroporation experiments. Some types of cells are better transfected in the form of square wave pulses, while others respond better to exponential decay forms.

5 Cell incubation time after electrotransfection

There is an optimal incubation time for each cell type after electrotransfection. For plasmid electrotransfection, the optimal incubation time is generally 4-48 hours, and the specific optimal time needs to be adjusted according to the experimental purpose, plasmid properties, and half-life of the expressed protein.

6 The issue of cell death in electrotransfection

When using EZ Transfer, due to the damage of electroporation to cells, according to experience, the transfection effect is best when the mortality rate after transfection is around 50%.

Related products

TR2000: Used for transfecting DNA into HEK293T cells.

Product Number: TR3091

Shipping and Storage

1. This product is transported at room temperature and stored for a long time at 4 ℃, with a validity period of 12 months.
2. This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ virus Transmission Kit is an enhancer developed and synthesized by our biological company for viral infections, which is synthesized using nanotechnology. The EZ virus transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ virus transmission kit not only significantly improves the efficiency of virus infection, but also has very low cytotoxicity and does not interfere with cellular physiological functions.

Note

1. After using the EZ virus Transmission kit for enhancement, the cell density during infection has an impact on the infection effect. It is recommended not to use excessively high cell density for infection.
2. Before using the EZ virus transmission kit enhancer, the optimal MOI (Multiplicity of Infection) of infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the expression level of the target protein. Different cells have their optimal MOI values. We need to conduct a MOI gradient experiment first to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The dilution solution for EZ virus transmission kit and virus is a serum-free liquid that is consistent with the cell base culture medium, such as serum-free DMEM or 1640.
4. The relationship between EZ virus transmission kit and infection efficiency. Under other fixed conditions, the larger the amount of enhancer used, the higher the infection efficiency. Generally, the amount of enhancer used can be 1-3 times the recommended amount. However, excessive use of enhancers may lead to cytotoxicity.

Other related reagents

• TR3012: Chronic virus infection enhancement reagent.
• TR3011: Adenovirus infection enhancing reagent.
• TR3003: Adenovirus infection enhancing reagent.
• TR3041: Retroviral infection enhancing reagent.
• TR3015: Virus infection enhancing reagent in animal bodies.

Product Number: TR3041

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ-RV Transmission Kit is an enhancer developed and synthesized by our company for retroviral infection, which is synthesized using nanotechnology. The EZ-RV Transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ-RV Transmission kit significantly improves the efficiency of retroviral infection while maintaining minimal cytotoxicity and not interfering with cellular physiological functions.

Note

1. After using the EZ-RV Transmission kit for enhancement, the degree of cell fusion during infection has an impact on the infection effect. It is recommended to conduct infection experiments when the degree of cell fusion is around 30-50%.
2. Before using the EZ-RV Transmission kit enhancer, the optimal MOI (Multiplicity of Infection) of infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the surface expression of the target protein. Different cells have their optimal MOI values. We need to conduct a MOI gradient experiment first to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The relationship between EZ-RV Transmission kit and infection efficiency. Under other fixed conditions, the larger the dosage of enhancer, the higher the infection efficiency. Generally, the dosage of enhancer can be used 1-3 times the recommended dosage. However, excessive use of enhancers may lead to cytotoxicity.

Other related reagents

• EnvirusTM-LV: Chronic virus infection enhancing reagent.
• EnvirusTM AAV: Adenovirus infection enhancing reagent.
• EnvirusTM-AV: Adenovirus infection enhancing reagent.
• EnvirusTM in vivo: A reagent for enhancing viral infection in animals.

Product Number: TR3015

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ virus in vivo kit is a nano polymer virus infection enhancement reagent developed and synthesized by our company. This reagent is synthesized using nanotechnology and enriches and protects virus particles through physical contact with them, assisting in their entry into cells, to achieve the goal of enhancing virus infection.

Features

1. Can be used for animal infection enhancement of adenoviruses, adeno-associated viruses, lentiviruses, and retroviruses.
2. On the basis of commonly using virus to infect animals, it can be pre mixed with enhancing reagents to achieve the goal of enhancing the ability of virus infection.
3. No obvious inflammatory response to animals, no involvement in physiological activities, and no impact on research.
4. Easy to operate.

Related reagents

• TR3041: Retroviral infection enhancing reagent.
• TR3012: Chronic virus infection enhancement reagent.
• TR3003: Adenovirus infection enhancing reagent.
• TR3011: Adenovirus infection enhancing reagent.

Product Number: TR3012

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ-LV Transmission Kit is an enhancer developed and synthesized by our company for chronic viral infections, which is synthesized using nanotechnology. The EZ-LV Transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ-LV Transmission kit significantly improves the efficiency of lentiviral infection while minimizing cell toxicity and not interfering with cellular physiological functions.

Note

1. After using the EZ-LV Transmission kit for enhancement, the cell fusion degree during infection has an impact on the infection effect. It is recommended to conduct infection experiments when the cell fusion degree is around 50% (adjust the suspension cells as needed, do not use too high cell density).
2. Before using the EZ-LV Transmission kit enhancer, the optimal MOI value (Multiplicity of Infection) for infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the expression level of the target protein. For cells with active division, such as Hela and 293 cells, when MOI=1-3, more than 80% of cells express the target gene. For non dividing cells, such as primary cells, the infection efficiency is low, and MOI gradient experiments are needed to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The dilution solution for EZ-LV Transmission kit and virus is a serum-free liquid that is consistent with the cell base culture medium, such as serum-free DMEM or 1640.
4. The relationship between EZ-LV Transmission kit and infection efficiency. Under other fixed conditions, the dosage of enhancer is within the range of 1-3 times the recommended dosage. The larger the dosage, the higher the infection efficiency.

Other related reagents

TR3012: Chronic virus infection enhancement reagent.

TR3003: Adenovirus infection enhancing reagent.

TR3041: Retroviral infection enhancing reagent.

TR3015: Virus infection enhancing reagent in animal bodies.

Product Number: TR3011

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ-AV Transmission Kit is an enhancer developed and synthesized by our company for adenovirus infection, which is synthesized using nanotechnology. The EZ-AV Transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ-AV Transmission kit significantly improves the efficiency of adenovirus infection while minimizing cell toxicity and not interfering with cellular physiological functions.

Note

1. After using the EZ-AV Transmission kit for enhancement, the degree of cell fusion during infection has an impact on the infection effect. It is recommended to conduct infection experiments when the degree of cell fusion is around 30-50%. If it is a suspended cell, the usual density of cells infected with adenovirus can be used.
2. Before using the EZ-AV Transmission kit enhancer, the optimal MOI (Multiplicity of Infection) of infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the surface expression of the target protein. Different cells have their optimal MOI values. We need to conduct a MOI gradient experiment first to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The dilution solution for EZ-AV Transmission kit and virus is a serum-free liquid that is consistent with the cell base culture medium, such as serum-free DMEM or 1640.
4. The relationship between EZ-AV Transmission kit and infection efficiency. Under other fixed conditions, the larger the dosage of enhancer, the higher the infection efficiency. Generally, the dosage of enhancer can be used 1-3 times the recommended dosage. However, excessive use of enhancers may lead to cytotoxicity.

Related reagents

• TR3012: Chronic virus infection enhancement reagent.
• TR3003: Adenovirus infection enhancing reagent.
• TR3041: Retroviral infection enhancing reagent.
• TR3015: Virus infection enhancing reagent in animal bodies.

Product Number: TR3003

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ-AAV Transmission Kit is an enhancer developed and synthesized by our company for adeno-associated virus infection, which is synthesized using nanotechnology. The EZ-AAV Transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ-AAV Transmission kit significantly improves the efficiency of virus infection while minimizing cell toxicity and not interfering with cellular physiological functions.

Note

1. After using the EZ-AAV Transmission kit for enhancement, the degree of cell fusion during infection has an impact on the infection effect. It is recommended to conduct infection experiments when the degree of cell fusion is around 50%.
2. Before using the EZ-AAV Transmission Kit enhancer, the optimal MOI (Multiplicity of Infection) of infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the surface expression of the target protein. Different cells have their optimal MOI values. We need to conduct a MOI gradient experiment first to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The dilution solution for EZ-AAV Transmission kit and virus is a serum-free liquid that is consistent with the cell base culture medium, such as serum-free DMEM or 1640.
4. The relationship between EZ-AAV Transmission Kit and infection efficiency. Under other fixed conditions, the larger the dosage of enhancers, the higher the infection efficiency. Generally, the dosage of enhancers can be used 1-3 times the recommended dosage. However, excessive use of enhancers may lead to cytotoxicity.
5. After using the EZ-AAV Transmission kit, sodium butyrate can also be used simultaneously.

Related reagents

• TR3011: Adenovirus infection enhancing reagent.
• TR3012: Chronic virus infection enhancement reagent.
• TR3041: Retroviral infection enhancing reagent.
• TR3015: Virus infection enhancing reagent in animal bodies.

Product Number: TR2000

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

TRANS2000 transfer reagent is a nano polymer transfection reagent developed and synthesized by our company. This reagent is synthesized using nanotechnology and is the latest generation of non viral transfection reagents. Due to the application of nanotechnology, TRANS2000 transfer reagent exhibits excellent low toxicity and high efficiency performance in cell transfection processes.

This product is recommended for transfection of conventional DNA and long segments (>1kb) of mRNA and other RNA.

Related reagents

• TR1000: Transfect small fragments of RNA (siRNA, miRNA, mimic, inhibitor, etc.) into animal cells.
• TR3015: Animal in vivo transfection of RNA and DNA.
• TR3091: Virus infection enhancement series reagents can enhance the infection efficiency of lentiviruses, adenoviruses, adeno-associated viruses, and retroviruses.

Product Number: TR1812

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EVO Transfer, DNA is a nano polymer transfection reagent developed and synthesized by our company. This reagent is synthesized using nanotechnology and is a new generation of non viral transfection reagents. Due to the application of nanotechnology, EVO Transfer, DNA exhibits excellent low toxicity and high efficiency performance during cell transfection.This product is used for in vivo transfection of DNA.

Features

The method is simple and fast, with no obvious inflammatory response to animals, and is safe for operators.

Application

1. In vivo studies of gene therapy in animals
2. In vivo studies on RNA interference in animals
3. In vivo study of protein function in animals

Related products

• TR1811: Used for in vivo transfection of RNA in animals.
• TR2000: Used to transfect long fragments of nucleic acid into cells.
• TR1000: Used to transfect short nucleic acids into cells.

Product Number: TR1811

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EVO Transfer, RNA is a nano polymer transfection reagent developed and synthesized by our company. This reagent is synthesized using nanotechnology and is a new generation of non viral transfection reagents. Due to the application of nanotechnology, EVO Transfer, RNA exhibits excellent low toxicity and high efficiency during cell transfection. This product is used for in vivo transfection of RNA.

Features

The method is simple and fast, with no obvious inflammatory response to animals, and is safe for operators.

Application

1. In vivo studies of gene therapy in animals
2. In vivo studies on RNA interference in animals
3. In vivo study of protein function in animals

Related products

• TR2000: Used for transfecting DNA into HEK293T cells.
• TR1000: Used for in vitro transfection of RNA into animal cells.