Product Number: BS03

Shipping and Storage

Store at -20°C.

Components

Description

WellStart III BST DNA polymerase is a hybrid enzyme containing WarmStafi Bst DNA polymerase and a thermostable reverse transcriptase. WarmStafi Bst III features modifications over Bst 3.0, eliminating nonspecific amplification during room-temperature reaction setup and eliminating the need for a separate activation step, thereby enhancing reaction specificity. The thermostable reverse transcriptase is a genetically engineered novel enzyme with significantly improved cDNA synthesis speed and thermal stability, capable of tolerating reaction temperatures up to 60°C, making it suitable for reverse transcription reactions with RNA templates possessing complex secondary structures. WellStart III BST DNA polymerase can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT-LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80 ℃ for 5 minutes to inactivate.

Product Number: PC11

Shipping and Storage

Ice pack transportation; Stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Components

Description

HS Taq DNA polymerase is a chemically modified Taq DNA polymerase, whose activity is completely blocked at room temperature and only released after heating at 95 ℃, which can prevent non-specific amplification and primer dimerization during sample preparation and reaction heating stages. Compared with antibody based hot start Taq enzymes, HS Taq DNA polymerase activity is more thoroughly blocked and has higher rigor; Compared with existing chemically modified hot start Taq enzymes, the activation time of HS Taq DNA polymerase is only 5 minutes, which is compatible with existing PCR programs. HS Taq DNA polymerase, combined with an optimized buffer system, can minimize non-specific amplification and primer dimers to the greatest extent possible, bringing extremely high sensitivity and specificity, making it very suitable for amplifying low copy genes from complex templates.

Application

Mainly used for DNA amplification reactions in animals, plants, and microorganisms. Hot start enzymes have 5 ‘-3’ exonuclease activity and can be used for fluorescence quantitative PCR reactions. The use of hot start amplification is a common method to improve PCR specificity, and hot start enzymes are a good choice. In addition, due to its high specificity and sensitivity, it is widely used in various fields such as constructing cDNA libraries, generating large amounts of DNA sequencing, mutant analysis and construction, gene isolation, genetic disease diagnosis, forensic identification, etc.

Unit definition

The activity of consuming 10 nmol of whole nucleotide as an acidic insoluble substance within 30 minutes at 74℃ using activated salmon sperm DNA as a template/primer is defined as one activity unit (U).

Product Number: PC06

Shipping and Storage

Ice pack transportation, stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Components

Description

Super Fidelity DNA Polymerase is a new generation of ultra fidelity DNA polymerase modified from Pfu DNA Polymerase, which has greatly improved its long segment amplification ability, amplification specificity, and amplification yield. By optimizing the reaction buffer and using simple templates such as lambda DNA and plasmids, fragments up to 40kb can be effectively amplified; Using complex templates such as genomic DNA, fragments up to 20kb can be amplified; The use of cDNA templates can effectively expand fragments up to 10kb in length. Its mismatch rate is 1/53 of that of ordinary Taq enzymes and 1/6 of that of Pfu enzymes, and the amplification speed can reach 15-30 seconds/kb. High fidelity and excellent amplification efficiency make Super Fidelity DNA Polymerase suitable for direct PCR of bacterial, fungal, plant tissue, animal tissue, or whole blood samples, with amplification products being flat ended.

Application

This product is suitable for PCR reactions using genomic DNA, cDNA, Plasmid DNA, and crude samples as templates.

1. High fidelity PCR and vector construction;
2. Gene cloning;
3. Gene directed mutagenesis;
4. High throughput PCR and sequencing.

Product Number: BS07

Shipping and Storage

Transportation under 0°C and storage at -25~ -15°C.

Component

Description

Bst DNA polymerase V2 is derived from Bacillus stearothermophilus DNA Polymerase I, which has 5´→ 3´ DNA polymerase activity and strong chain replacement activity, but no 5´→3´ exonuclease activity. Bst DNA Polymerase V2 is ideally suitable for strand-displacement, isothermal amplification LAMP (Loop mediated isothermal amplification) and rapid sequencing. Well Start Bst DNA polymerase,large fragment is a hot-start version based on Bst DNA polymerase V2 obtained by reversible modification technology, which can inhibit DNA polymerase activity at room temperature, so the reaction system can be operated and formulated at room temperature to prevent non-specific amplification and improve reaction efficiency, and this version can be lyophilized. In addition, its activity is released at high temperatures, so there is no need for a separate activation step.

Application

1. LAMP isothermal amplification;
2. DNA strand single displacement reaction;
3. High GC gene sequencing;
4. DNA sequencing of nanogram level.

Unit Definition

One unit is defined as the amount of enzyme that incorporate 25nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Heat Inactivation

80℃, 20 min.

Product Number: PC18

Shipping and Storage

Storage at -20℃.

Component

Description

The Klenow Fragment, also known as the Klenow fragment, is a large fragment of Escherichia coli DNA polymerase I (E. coli DNA polymerase I). It retains the 5’→3′ polymerase activity and 3’→5′ exonuclease activity of DNA polymerase I but lacks the complete 5’→3′ exonuclease activity of the full Klenow enzyme. The 3’→5′ exonuclease activity of the Klenow Fragment ensures the fidelity (proofreading) of DNA synthesis.

Application

Fill in at the 5 ‘overhang end of double stranded DNA; Flattening (also known as flattening) the 3 ‘overhang of double stranded DNA; 5 ‘protruding end mark; Random primer method for DNA labeling; Sanger deoxygenation method for DNA sequencing; The synthesis of the second strand of cDNA or site directed mutagenesis reaction.

Features

For adhesive ends with 5 ‘or 3’ protrusions, it can catalyze the generation of flat ends for subsequent flat end connections.

Product Number: PC0302

Shipping and Storage

-20°C

Component

Description

Fast Pfu DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. This enzyme is modified from other high fidelity enzymes, with strong amplification ability, fast amplification speed, and high fidelity. It overcomes the shortcomings of poor amplification ability, low yield, and slow amplification speed of ordinary Pfu enzymes, greatly shortening the reaction time. This product can be used for long fragment amplification and amplification of various complex templates. The 3 ‘end of the PCR product obtained from amplification does not contain the “A” base and can be directly cloned into a flat end vector. If T/A cloning is required, “A” needs to be added to the end of the PCR product for cloning. This product is suitable for gene cloning, gene site directed mutagenesis, SNP and other amplification experiments.

Definition of Activity

The amount of enzyme required to incorporate 10nmol of deoxyribonucleotides into acidic insoluble substances within 30 minutes at 74℃ is defined as 1 active unit (U).

Quality control

After multiple column purifications, SDS-PAGE analysis showed that its purity was greater than 98%; No exogenous nuclease activity detected; After being stored at room temperature for one month, there was no significant change in activity.

Pfu DNA Polymerase, 5U/μL

Product Number: PC0301

Shipping and Storage

-20°C

Component

Description

This enzyme is a high fidelity heat-resistant DNA polymerase, with a fidelity 8-10 times that of Taq DNA polymerase. This enzyme not only has 5′-3′DNA polymerase activity, but also has 3′-5′exonuclease proofreading activity, which can timely remove nucleotide mismatches in the DNA synthesis chain, greatly increasing the accuracy of base pairing and ensuring the high fidelity of DNA synthesis. This enzyme does not have 5′-3′exonuclease activity.

Source

E. The recombinant strain of Escherichia coli contains genes cloned from Pyrococcus furiosus.

Definition of Activity

The amount of enzyme that can catalyze the polymerization of 10nmol dNTP into a nucleotide fragment by reacting calf thymus DNA as a template at 72℃ for 30 minutes is defined as one active unit (U).

Storage buffer

 [PH8.0] 20mM Tris HCl, 100mM KCl, 0.1mM EDTA, 0.1% NP-40 (v/v), 0.1% Tween-20 (v/v), 1mM DTT, 50% glycerol (v/v).

Reaction buffer

[10 × Pfu Buffer (containing Mg²⁺）] 200mM Tris-HCl（PH 8.8）, 100mM KCl, 160mM(NH4)2SO4，1 % Triton X-100，1 mg/ml BSA, 20mM MgSO4.

Product Number: PH29P

Shipping and Storage

-20℃.

Components

Description

Phi29 Plus DNA polymerase is a protein engineered Phi29 DNA polymerase that is expressed in Escherichia coli and purified and isolated multiple times. Compared with wild-type Phi29 DNA polymerase, it has higher amplification efficiency and sensitivity, and can greatly shorten reaction time. Phi29 plus DNA polymerase has special chain displacement activity and efficient continuous synthesis properties, with strong binding ability to templates. It can continuously synthesize up to 70kb of DNA fragments without dissociating from the template. At the same time, the enzyme has a strong 3´→5´exonuclease correction function, with a fidelity 100 times higher than Taq DNA polymerase.

Application

1. Thermostatic protein prime DNA amplification;
2. Using random primers to amplify DNA;
3. Roll ring replication;
4. Copy extended region.

Features

1. Extremely high infiltration rate;
2. Super strong chain replacement capability;
3. High fidelity;
4. Reaction at moderate temperature;
5. Extremely high sensitivity and amplification efficiency.

Product Number: RNK35H200

Shipping and Storage

-20℃

Components

Description

This product is a recombinant murine RNase inhibitor expressed and purified in Escherichia coli, with a molecular weight of approximately 50 kD. It can specifically inhibit the activity of RNase A, B, and C,Can form a 1:1 complex with RNase, thereby inhibiting its activity. This reaction is reversible, as urea and thiol reagents can dissociate the complex, causing RNase to be renaturated while inhibitors are irreversibly deactivated. When in use, it can be directly added to the reaction solution containing RNA. This product belongs to protein properties and is different from other competitive inhibitors (nucleic acids, inorganic phosphates). It can be easily removed from the reaction system by phenol treatment.

Compared with human RNase inhibitors, murine RNase inhibitors have higher antioxidant activity and are more suitable for experiments with high DTT sensitivity.

This product does not contain ingredients such as glycerol that affect the freeze-drying process, and can be used for the preparation of freeze-drying reaction systems and product design.

Application

This product can be used in any experiment that requires avoidance of RNase interference to prevent RNA degradation, such as:

1. CDNA synthesis reaction.
2. External translation.
3. Polyribosome separation.
4. There is no cellular system transcription in vitro.

Unit definition

The amount of enzyme required to inhibit 50% of 5ng RNase A activity is defined as one activity unit (U). The activity of RNase A was quantitatively determined by inhibiting the hydrolysis of Cyclic2 ‘, 3’ – CMP to generate 3 ‘- CMP

Quality control

After multiple column purifications, SDS-PAGE gel detection showed only a clear and single target band; The qPCR method detects no residual E. coli DNA and no contamination of nucleic acid endonucleases.

Product Number: PC608

Shipping and Storage

-20°C.

Components

Description

Hot Exo- DNA Polymerase purified self weight group E. Coli strain, this enzyme is a natural enzyme obtained through genetic engineering modification.This polymerase is a high-fidelity heat-resistant DNA polymerase with a half-life of 8 hours at 100℃. Suitable for primer extension and high-temperature (72℃) DNA sequencing.

Unit Definition

The amount of enzyme required for the incorporation of 10 nmol of whole nucleotides into acid insoluble precipitates within 30 minutes at 75℃ is defined as 1 active unit (U).

Quality control

The purity of Hot Exo- DNA Polymerase SDS-PAGE is greater than 99%, and there is no activity of endonuclease or exonuclease.