Specifications and Analysis

The following parameters represent the standard quality profile for this product based on the Certificate of Analysis:

Storage and Stability

Retest Interval: 24 months from the date of production when stored under recommended conditions.
Handling: Keep container tightly closed in a dry and well-ventilated place.
Safety: Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.

25mM each, Purity>98%

dATPαS, dGTPαS, dCTPαS and dTTPαS at each 25mM in the mixture

Product Number: P1331

Storage

Poly A RNA carrier is temperature stable, can ship at room temperature. Store at -20℃, Keep away from sunlight, sealed in dry place.

Description

Poly A is a synthetic nucleic acid sequence, which increase the yield of RNA or DNA during extraction process. Ethanol precipitation is commonly used to recover DNA and RNA from liquid samples. However, ethanol precipitation cannot completely recover the nucleic acid from the sample, at least 30% of the nucleic acid will lost. If the concentration of nucleic acid in the liquid sample is very low or DNA < 200bp, only 50% DNA and RNA can be recovered by ethanol precipitation. Poly A carrier is a type of synthetic polymer. Adding Poly A carrier in ethanol precipitation can significantly improve the yield of nucleic acid precipitation, and the recovery rate of trace DNA can reach 95-98%. At the same time, short primer (< 22bp) fragments and dNTP can be selectively removed for precipitation and recovery of labeled probes, and untagged dNTP can be removed. Compared to biologically derived nucleic acid precipitation aids such as glycogen and tRNA, Poly A carrier itself is free of nucleic acid pollution, DNase and RNase activity, and does not affect digestion, connection, transcription, PCR, transformation and transfection, nor does it affect DNA electrophoresis and DNA protein interaction. Poly A carrier has become the most commonly used nucleic acid precipitant.

Package

1g, 5g, or bulk.

Working concentration

A concentration of 0.5 mg/ml is recommended.

Features

• 1. Significantly improve the yield of DNA or RNA precipitation.
• 2. The recovery of trace DNA and RNA (20pg) was 95-98%.
• 3. It does not affect the enzyme cutting, linking, transcription, PCR and other reactions.
• 4. It does not affect the interaction between electrophoresis and DNA protein.

Note

Poly A carrier will increase the optical density value of RNA or DNA. Thus, when measuring the optical density value, to eliminate the influence of Poly A carrier, a blank control sample should be made according to the same experimental process (use the same reagent and Poly A carrier, but do not contain RNA or DNA samples, and dissolve the final Poly A carrier precipitation in the same solution as the sample). The optical density values of sample and blank control were measured at 260 and 280 nm. The actual optical density of the sample can be obtained by subtracting the optical density of the blank control from that of the sample. If the quantification does not need to be very accurate, it can also be estimated.

Product Number: DN32-HS

Shipping and Storage

Stored at -20℃, with a shelf life of 1 year.

Description

Hot Start dNTP Mixture is a dNTP product that has undergone special chemical modifications. Under normal circumstances, Hot Start dNTP Mixture cannot participate in the generation of phosphodiester bonds. Only after heat shock, Hot Start dNTP Mixture will become a regular dNTP and participate in PCR amplification. This product can significantly reduce non-specific amplification caused by non-specific annealing or primer dimers.

Protocol

1. This product is sensitive to temperature and should be avoided from repeated freezing and thawing. It is recommended to pack it in advance before use and thaw it on ice during use.
2. This product begins to show a thermal activation trend at 50-60℃, and when the temperature reaches 95℃, heating for 30-60 seconds can complete the thermal activation.

Note

1. Hot Start dNTP Mixture can be stored at 4℃ for up to 15 days, but it is not recommended to store at room temperature.
2. When using cDNA as a template, it is recommended to use commercially available purification kits to remove unadulterated nucleotides. If the experimental protocol requires the use of unpurified cDNA products, the reaction volume of cDNA synthesized products in the PCR system should not exceed 1/10.
3. In the multiplex reaction of amplifying four or more targets, it is recommended to try adding KCl with a final concentration of up to 100mM to improve the results. The KCl content in the PCR buffer should be considered during the optimization process.

dNTP

Fluorescein-12-dUTP 1mM Sodium solution

Description

Fluorescein-12-dUTP is recommended for direct enzymatic labeling of DNA, cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA, cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2′-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution. Fluorescein-12-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment and DNA Polymerase I.

Applications

Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.

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