Product Number: PC0302

Shipping and Storage

-20°C

Component

Description

Fast Pfu DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. This enzyme is modified from other high fidelity enzymes, with strong amplification ability, fast amplification speed, and high fidelity. It overcomes the shortcomings of poor amplification ability, low yield, and slow amplification speed of ordinary Pfu enzymes, greatly shortening the reaction time. This product can be used for long fragment amplification and amplification of various complex templates. The 3 ‘end of the PCR product obtained from amplification does not contain the “A” base and can be directly cloned into a flat end vector. If T/A cloning is required, “A” needs to be added to the end of the PCR product for cloning. This product is suitable for gene cloning, gene site directed mutagenesis, SNP and other amplification experiments.

Definition of Activity

The amount of enzyme required to incorporate 10nmol of deoxyribonucleotides into acidic insoluble substances within 30 minutes at 74℃ is defined as 1 active unit (U).

Quality control

After multiple column purifications, SDS-PAGE analysis showed that its purity was greater than 98%; No exogenous nuclease activity detected; After being stored at room temperature for one month, there was no significant change in activity.

Pfu DNA Polymerase, 5U/μL

Product Number: PC0301

Shipping and Storage

-20°C

Component

Description

This enzyme is a high fidelity heat-resistant DNA polymerase, with a fidelity 8-10 times that of Taq DNA polymerase. This enzyme not only has 5′-3′DNA polymerase activity, but also has 3′-5′exonuclease proofreading activity, which can timely remove nucleotide mismatches in the DNA synthesis chain, greatly increasing the accuracy of base pairing and ensuring the high fidelity of DNA synthesis. This enzyme does not have 5′-3′exonuclease activity.

Source

E. The recombinant strain of Escherichia coli contains genes cloned from Pyrococcus furiosus.

Definition of Activity

The amount of enzyme that can catalyze the polymerization of 10nmol dNTP into a nucleotide fragment by reacting calf thymus DNA as a template at 72℃ for 30 minutes is defined as one active unit (U).

Storage buffer

 [PH8.0] 20mM Tris HCl, 100mM KCl, 0.1mM EDTA, 0.1% NP-40 (v/v), 0.1% Tween-20 (v/v), 1mM DTT, 50% glycerol (v/v).

Reaction buffer

[10 × Pfu Buffer (containing Mg²⁺）] 200mM Tris-HCl（PH 8.8）, 100mM KCl, 160mM(NH4)2SO4，1 % Triton X-100，1 mg/ml BSA, 20mM MgSO4.

Product Number: NG0105

Shipping and Storage

Store at -20℃ and transport on dry ice.

Components

Description

Tn5 transposase is a highly active mutant of wild-type Tn5 transposase, which can efficiently insert Tn5 transposons into target sequences. Tn5 transposase recognizes the inner end (IE), outer end (OE), and chimeric end (ME) sequences of Tn5 transposase sequences, with the highest in vitro transposition efficiency achieved by fragments containing ME sequences. The insertion site of Tn5 transposase has high randomness, making it widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and second-generation sequencing library construction.

Application

In vitro genetic modification (integration of exogenous genes into host cells), high-throughput sequencing, CUT&Tag.

Product source

Escherichia coli strain containing Tn5 transposase sequence.

Stock solution

50mM Tris-HCl (pH 8.0 @ 25°C), 100mM NaCl, 0.1mM EDTA, 0.1% Triton X-100, and 50% glycerol (V/V).

Unit Definition

One unit of Tn5 transposase refers to the amount of enzyme required to completely cleave 1 μ g of DNA fragments containing recognition sequences under 37°C conditions for one hour.

Product Number: EK0513

Shipping and Storage

Store at -20℃ and transport on dry ice.

Components

Description

The second-generation sequencing library end repair kit (Illumina) provides the enzyme end repair enzymes and reaction buffer required for constructing DNA libraries. It can repair the ends of mechanically processed fragmented DNA or small fragments, forming flat ends of DNA double strands. The resulting product does not require purification and can be directly used for Adaptor ligation of DNA fragments using the second-generation sequencing library connection kit. Easy to operate, higher efficiency in library conversion.

Product Number: LG02

Shipping and Storage

Store at -30～-15 ℃ and transport at ≤ 0℃.

Components

Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between adjacent 5′- phosphate and 3′ – hydroxyl ends on double stranded DNA or RNA. This enzyme can not only catalyze connections between flat or sticky ends, but also repair single strand cleavage in double stranded DNA, RNA, or DNA/RNA hybrid double strands.

Application

1. The connection between DNA fragments and carrier DNA.
2. The connection between DNA fragments and Linker or Adaptor DNA.

Unit definition

In a 20μl linkage reaction system, when 6μg of λDNA-Hind III decomposition product was reacted at 16℃ for 30 minutes, more than 50% of DNA fragments were linked, and the required enzyme quantity was defined as one active unit (U).

Product Number: PCM14

Shipping and Storage

-20°C; try to avoid repeated freeze-thaw cycles.

Components

Description

Multiplex Pro MasterMix is a premixed system suitable for various types of multiple PCR, with a concentration of 2.5×, contains DNA polymerase, PCR buffer, dNTPs, Mg²⁺ and components such as stabilizers and enhancers, this product can be amplified by adding primers and templates. The operation is simple and convenient, reducing the probability of contamination, and improving detection flux and reproducibility.

The DNA polymerase contained in Multiplex Pro MasterMix is a genetically engineered recombinant enzyme with 5’→ 3′ DNA polymerase activity and no 5’→3′ exonuclease activity; DNA polymerase, modified by a new type of antibody, is an antibody modified hot start enzyme that can effectively reduce non-specific amplification generated by non-specific binding or dimerization of primers and templates at room temperature. It also has excellent characteristics such as short activation time, strong amplification ability, high sensitivity, and good stability.The unique combination of PCR buffer system and hot start enzyme significantly improves the amplification efficiency of PCR, with higher sensitivity and stronger inhibitor tolerance.

Multiplex Pro MasterMix has a wide range of applications and is suitable for various types of multiplex PCR, such as microsatellite analysis, amplification library construction, genotyping, and SNP detection.

Notes

1. Before use, please gently mix the product upside down after it has completely melted, and centrifuge briefly before use.
2. Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored at -20℃ for a long time.

Product Number: PCM313

Shipping and Storage

-20°C; For frequent uses, store at 2-8°C.

Components

Description

This product is a premixed system composed of Kfu DNA Polymerase, Mg²⁺, dNTPs, PCR stabilizers and enhancers, with a concentration of 2×。Kfu DNA Polymerase is a fast and highly efficient high-fidelity DNA polymerase with 5′-3′ DNA polymerase activity and 3′-5′ exonuclease activity. It has advantages such as strong amplification ability, high fidelity, and strong specificity.2× Mix has added unique amplification enhancing factors and elongation factors, and the unique formula makes the entire reaction system very stable and easy to operate, suitable for amplification of various fragments and templates.This product is suitable for gene cloning, second-generation library building amplification, gene directed mutation, SNP and other amplification experiments.

Quality Control

After testing, there is no exogenous nuclease activity, which can effectively amplify various templates; Stored at 2-8℃ for one month, there was no significant change in activity.

Product Number: PCK48

Shipping and Storage

Box 1 -20℃ storage, dry ice transportation.

Box 2 2-8℃, transported in ice bags

Components

• Box 1

• Box 2

Description

The Universal DNA Library Kit (Illumina&MGI) is a second-generation sequencing enzyme digestion library kit developed for Illumina and MGI sequencing platforms. It includes three modules: DNA fragmentation/end repair/addition of A, adapter connection, and pre mixed enzymes required for library enrichment in library construction.By controlling the reaction time, sample DNA fragments from different sources such as 0.1ng-1μg genomic DNA, PCR amplification products, and FFPE can be reduced to small fragments, avoiding tedious ultrasound processes and instrument dependence.This kit simplifies the purification steps and shortens the library construction time. In the amplification module, high fidelity DNA polymerase is used for library enrichment, and PCR amplification is performed without preference, ensuring the accuracy of sequencing results.

Features

1. Accurate enzyme digestion, fragment end repair and A tube completion, without the need for purification and direct connection to the connector.
2. After connecting the joint, use matching magnetic beads for direct sorting (optional).
3. High fidelity enzymes for PCR enrichment and amplification minimize amplification preference.
4. Suitable for building libraries for different species such as humans, animals, plants, microorganisms, etc. The resulting library is applicable to various platforms such as Illumina and MGI.

Schematic diagram of DNA database construction process

DNA library construction process

Please read this operating instruction carefully before the experiment and choose the operating plan based on the type of sequencing platform used. Before the experiment starts, clarify the nucleic acid concentration, and the input amount of this reagent kit is 0.1ng-1μg. The recommended sample size is 1ng-500ng DNA. DNA solution does not contain chelating agents, if DNA dissolves in 1×TE or EDTA containing solutions, it is recommended to use magnetic beads for purification or add Neutralization Reagent.

Product Number: PCK47

Shipping and Storage

-20°C

Components

Description

Super Fi Amplification Mix for NGS is a PCR amplification premix that combines fidelity and amplification specificity, suitable for PCR amplification of high-throughput sequencing libraries.2×Super HiFi PCR Mix is a 2×premixed system composed of High-Fidelity DNA Polymerase, dNTP, PCR stabilizers and enhancers, which has advantages such as strong amplification ability, high fidelity, and strong specificity.When performing library amplification reactions, a wide range, no preference, and high yield of library amplification can be achieved, ensuring the accuracy of sequencing results. During the operation, only primers and templates need to be added, simplifying the experimental steps, improving the experimental throughput and repeatability of the results.2×Mix has added unique protective agents, amplification enhancers, and elongation factors. The unique formula makes the entire reaction system very stable and suitable for amplification of different template libraries.

Note

1. It is recommended to use high-quality purified templates and operate according to the instructions.
2. The number of cycles for library amplification is set based on the input amount. If the number of cycles is too low, it will lead to a low concentration of outbound products, while if it is too high, there will be a preference for amplification.
3. Please perform all operations on ice, 2 × After thawing, please thoroughly mix the Super HiFi PCR Mix and store it at -20 ℃ in a timely manner after use.

Product Number: FER46

Shipping and Storage

Store at -20℃ and transport on dry ice.

Components

Description

Onestep DNA Frag And ER Reagent is a new generation of enzyme digestion repair reagents developed for Illumina and MGI high-throughput sequencing platforms. It can complete one-step fragmentation/final repair/addition of A. This product includes FER Enzyme Mix and FER Buffer, which can achieve fragmentation and end repair in one tube, avoiding cumbersome ultrasound processes and instrument dependence, and is easy to operate. It can achieve efficient and fast fragmentation, end repair, and dA tail addition for different DNA samples, and is applied to downstream library construction. It has the advantages of low preference, stable enzyme digestion repair effect, and higher library transformation efficiency. It can be applied to fragment, end repair, and A addition reactions of samples from different sources such as 0.1 ng-1ug genomic DNA, PCR amplification products, FFPE, etc.