Product Number: RE05871

Shipping and Storage

-20℃.

Components

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Product Number: REXM01

Shipping and Storage

Stored at -20℃, valid for two years.

Components

Description

Our company’s XmaI rapid endonuclease is a series of high-quality restriction endonucleases that have been genetically engineered and can quickly complete DNA cleavage using only one buffer within 5-15 minutes.

Application

Suitable for rapid enzymatic digestion of plasmid DNA, PCR products, or genomic DNA.

Features

1. Enzyme digestion can be completed within 5-15 minutes
2. This series of endonucleases share a common enzyme cleavage buffer FlashCut™Buffer, Greatly simplifying the enzyme digestion reaction system, facilitating dual enzyme digestion or multi enzyme digestion;
3. Targeting different enzymes in FlashCut™  The problem of differences in activity in Buffer has been addressed by adjusting the concentrations of different enzymes. Therefore, the enzyme digestion reaction can be carried out uniformly at a dosage of 1μL enzyme per 20μL system;
4. Alkaline Phosphatase, Antarctic Phosphatase, T4 DNA Ligase, T4 Polynucleotide Kinase, T4 PNK (3′ phosphatase minus) Many modifying enzymes are 100% compatible with FlashCut™ Buffer enables compatibility between reaction systems such as’ enzyme cleavage linking ‘and’ enzyme cleavage modification linking ‘, supporting one tube reactions;
5. Good enzyme activity redundancy makes it easy to cope with substrate excess or difficult template enzyme digestion.

Product Number: RESA04

Shipping and Storage

Stored at -20℃, valid for two years.

Component

Description

The rapid endonucleases produced by our company are a series of high-quality restriction endonucleases that have undergone genetic engineering recombination and can quickly complete DNA enzyme cleavage within 5-15 minutes using only one buffer solution.

Application

SapI rapid endonuclease is suitable for rapid enzymatic digestion of plasmid DNA, PCR products, or genomic DNA.

Features

1. Enzymatic cleavage can be completed within 5-15 minutes;
2. All endonucleases share one enzyme digestion buffer, CutEZ™ Buffer, Greatly simplifies the enzyme digestion reaction system, making it convenient for double enzyme digestion or multi enzyme digestion;
3. Targeting different enzymes in CutEZ™ The problem of differences in activity in Buffer has been addressed by adjusting the concentrations of different enzymes. Therefore, the enzyme digestion reaction can be carried out uniformly at a dosage of 1μL enzyme per 20μL system;
4. Alkaline Phosphatase, Antarctic Phosphatase, T4 DNA Ligase, T4 Polynucleotide Kinase, T4 PNK (3′ phosphatase minus) Many modified enzymes are 100% compatible with CutEZ ™ Buffer, Make reaction systems such as’ enzyme cleavage linking ‘and’ enzyme cleavage modification linking ‘compatible and support one tube reactions;
5. Good enzyme activity redundancy makes it easy to cope with substrate excess or difficult template enzyme digestion.

Product Number: RE0585

Shipping and Storage

-20℃.

Components

Description

SspDI belongs to the conventional restriction enzyme series and can recognize G ^ GCGCC sequences. Unlike the Thunder series of rapid endonucleases, SspDI requires a longer time for enzyme cleavage to achieve complete cleavage of the DNA substrate, but this enzyme still uses the Thunder restriction enzyme universal reaction buffer FlashCut™ Buffer ， Can achieve dual enzyme digestion.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0584

Shipping and Storage

-20℃.

Components

Description

BstXI belongs to the Type IIP restriction enzyme and recognizes palindrome sequences. The optimized reaction buffer maximizes the functionality of BstXI, while the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.

Suggested reaction conditions

1. 1×Cut Buffer C;
2. Incubate at 37℃;
3. Prepare the reaction system according to the “DNA enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0583

Shipping and Storage

-20℃.

Components

Description

BspQI belongs to the Type IIS restriction enzyme, which recognizes non palindromic sequences and performs cleavage outside of the recognized sequence. It is commonly used for Golden Gate assembly. The optimized reaction buffer maximizes the functionality of BspQI, while the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.

Suggested reaction conditions

1. 1×HN buffer solution;
2. Incubate at 50℃;
3. Prepare the reaction system according to the “DNA enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0581

Shipping and Storage

-20℃.

Components

Description

BsmBI belongs to the Type IIs restriction enzyme, which can recognize non palindromic sequences and perform cleavage outside of the recognized sequence. It is commonly used for Golden Gate assembly. The optimized reaction buffer maximizes the functionality of BsmBI, while the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.

Suggested reaction conditions

1. 1 x HN buffer solution;
2. Incubate at 55℃;
3. Prepare the reaction system according to the “DNA enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0580

Shipping and Storage

-20℃.

Components

Description

BsiWI belongs to the Type IIP restriction enzyme and recognizes palindrome sequences. The optimized reaction buffer maximizes the functionality of BsiWI, while the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.

Suggested reaction conditions

1. 1×Cut Buffer C;
2. Incubate at 56℃;
3. Prepare the reaction system according to the “DNA enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0579

Shipping and Storage

-20℃.

Components

Description

SgeI can cleave DNA targets containing 5-methylcytosine on single or double stranded DNA. SgeI restriction endonuclease can recognize the m5CNNG (9/13) ^ site and has the best cleavage effect at 37°C in its unique buffer. To ensure consistent performance, the enzyme storage buffer contains pre mixed BSA, which enhances enzyme stability and binds to potential contaminants in DNA preparations.

Suggested reaction conditions

1. 1×SgeI buffer solution;
2. Incubate at 37℃;
3. Prepare the reaction system according to the “DNA enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0578

Shipping and Storage

-20℃.

Components

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.