Product Number: CK001

Shipping and Storage

Store at -20°C or below to avoid repeated freeze-thaw cycles. The validity period is 6 months.

Description

This document describes the recommended usage instructions for the Human NK Cell Expansion Kit for NK cell expansion. The kit contains three lyophilized products: K001-1A, K001-2A, and K001-3A. This kit is designed to be used with an initial culture medium. The specific usage recommendations are as follows:

Product Reconstitution

CK001-1A: Dissolve the lyophilized powder in 100μL of water, then add 7.4mL of PBS to prepare a coating solution; total volume is 7.5mL. Store at -20°C or below.
CK001-2A: Dissolve the lyophilized powder in 100μL of water, then add 9.9mL of initial culture medium to prepare an activation factor; total volume is 10mL. Store at -20°C or below.
CK001-3A: Dissolve the lyophilized powder in 270μL of water, then add 9.73mL of initial culture medium to prepare an expansion factor; total volume is 10mL. Store at -20°C or below.

Usage summary table

Product Number: CEK004

Shipping and Storage

Store at -20°C . Unused factors should be stored in a -20°C freezer and thawed in a 4°C refrigerator when needed.

Component

Description

This kit is designed for the highly efficient activation and large-scale expansion of human natural killer (NK) cells in vitro. By integrating critical signaling pathway factors essential for NK cell activation with key cytokines crucial for maintaining cell viability and function, it enables the rapid production of high-purity, highly active NK cells from both frozen and fresh peripheral blood mononuclear cells (PBMCs) or cord blood mononuclear cells (CBMCs). It serves as a powerful tool for tumor immunotherapy research.

Application

1. NK cell research for cancer immunotherapy
2. CAR-NK cell therapy development
3. Functional and biological studies of NK cells
4. Large-scale preparation of NK cells for in vitro cytotoxicity assays

Features

1. High Expansion Efficiency: Proprietary formulation of cytokines and activation factors supports up to approximately 1000-fold expansion of NK cells within 18 days.
2. High Target Cell Purity: The optimized system effectively promotes the specific proliferation of NK cells, yielding a final population of high purity.
3. Excellent Media Compatibility: Compatible with a wide range of common basal media, offering flexibility for your experimental protocol.
4. Defined, Serum-Free Formulation: Uses a pure protein factor approach, ensuring consistency, reliability, and enhanced safety by eliminating variability associated with serum.

Product Number: CK02501

Characteristics:

Source: Chinese Hamster Ovary cell line

Description: Human IL-10(Ser19-Asn178)

Accession #P22301

Predicted molecular mass: 18.6kDa

Specification:

Appearance: White powder,colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a cell proliferation assay using MC-9 mouse mast cells. The ED₅₀ is ≤1ng/mL.
Formulation: Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size: 10μg/vial, 50μg/vial, 1mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at -20°C to -80°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Product Number: CK12441H

Characteristics:
Source: HEK293
Description: Human IGF-II(Ala25-Glu91), Accession # P01344.1
Predicted molecular mass: 7.5kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is ≤50 ng/mL.
Formulation: Lyophilized from a 0.2 μm filtered solution containing TFA, ACN, 5% Trehalose.
Size: 10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in 4mM HCl.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Product Number: CK12101

Characteristics:
Source: Chinese Hamster Ovary cell line
Description: Human PDGF-AB (Ser87-Thr211 & Ser82-Thr190) Accession # P04085 & P01127
Predicted molecular mass: 14.3 kDa & 12.3 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a cell proliferation assay using NIH3T3 cells. The ED50 is ≤ 1.0 ng/ml.
Formulation: Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH 7.4.
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Product Number: CK11742L

Characteristics:
Source: HEK293
Description: Human Vitamin D BP (Leu17-Leu474, K436T), with a C-terminal 6-His tag
Accession # P02774
Predicted molecular mass: 52.0 kDa

Specification:
Appearance: Colorless clear liquid
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤100 EU/mg by the LAL method
Formulation: 0.22 μm-filtered solution containing PBS, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Shipping: The product is shipped with blue ice.
Storage & Stability: 24 months at -20°C to -80°C under sterile conditions; 6 months at 2°C to 8°C under sterile conditions. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Product Number: CK11541H

Characteristics:

Source: HEK293
Description: Human 4-1BBL (Arg71-Glu254)
Accession # P41273.1
Predicted molecular mass: 19.4 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤50 EU/mg by the LAL method
Activity: Measured by its ability to induce IL-8 secretion in PBMCs.
Formulation: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH 7.4
Size: 10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Product Number: CK11442H

Characteristics:

Source: HEK293
Description: Human Factor IX (Thr29-Thr461), with a C-terminal 6-His tag. Accession # P00740
Predicted molecular mass: 49.5 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
Formulation: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Product Number: CK11242M

Characteristics:

Source: HEK293
Description: Mouse IL-27 (Y19-P228 & F29-S234), with a C-terminal 6-His tag Accession # O35228 & Q8K3I6
Predicted molecular mass: 48.8 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a VSV inhibition assay with Wish cells. The ED50 is ≤1 ng/ml.
Formulation: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitol and 0.1% CHAPs, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Product Number: CK11142H

Characteristics:

Source: HEK293
Description: Human IL34 (Asn21-Pro242), with a C-terminal 6-His tag. Accession # NP_689669
Predicted molecular mass: 25.2 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a cell proliferation assay using human PBMCs. The ED50 for this effect is ≤10 ng/mL.
Formulation: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.