Description

AacCas12b nuclease (C2c1) is an RNA-mediated endonuclease that specifically shears target double-stranded DNA in the presence of PAM (TTN), causing DNA double-strand breaks and sticky ends. In contrast, AacCas12b specifically shears single-stranded DNA targets independently of the PAM sequence. In addition, either double-stranded or single-stranded DNA targets can activate the trans-shearing activity of AacCas12b (i.e., bypass shearing activity/accessory shearing activity), i.e., when the AacCas12b enzyme binds to sgRNA and target DNA to form a ternary complex, it is activated to trans-shear the non-specific sequence ssDNA, shredding any sequence of ssDNA in the system.

AacCas12b is derived from the acidophilic thermotolerant bacterium Alicyclobacillus acidoterrestris, and the optimal shearing reaction temperature is 48°C.

AacCas12b is dependent on crRNA and tracrRNA (or sgRNA formed after ligation) for guidance. In addition, AacCas12b can be applied not only for the shearing of target dsDNA, but its trans-shearing activity against ssDNA can also be used for the rapid detection of target nucleic acids.

Component

Storage condition

Store at -30 ~ -15 ℃. Transportation conditions: ≤ 0 ℃.

Unit Definition

1unit refers to the amount of Cas12b enzyme required to cleave 1 pmol ssDNA probe within 1 minute under reaction conditions of 48 ℃.

Source

Obtained through E. coli recombination, expression, and purification, the expressed gene is derived from Alicyclobacillus acidoterrestris.

Applications

Used for the cleavage of target dsDNA, the trans cleavage activity of ssDNA can also be used for rapid detection of target nucleic acids.

Description

LwaCas13a Nuclease (C2c2) is a crRNA mediated RNA endonuclease derived from Leptotrichia wadei strain. In the presence of PFS sequences in the target single stranded RNA, it can specifically recognize and cleave the target RNA. In addition, Cas13a also has trans cleavage activity (i.e., bypass cleavage activity/accessory cleavage activity), which means that when LwaCas13a protein binds to crRNA and target RNA to form a ternary complex, it is activated for trans cleavage activity against non-specific sequence single stranded RNA, shredding any sequence single stranded RNA in the system. This activity has also been used in the development of rapid detection kits for target nucleic acids.

Component

Storage condition

Store at -30 ~ -15 ℃. Transportation conditions: ≤ 0 ℃.

Unit Definition

1 unit refers to the amount of Cas13a enzyme required to cleave 1 pmol ssRNA probe within 1 minute under 37 ℃ reaction conditions.

Source

Obtained through E. coli recombination, expression, and purification, the expressed gene is derived from Leptotrichia wadei.

Applications

Cut any sequence of single stranded RNA, etc.

Description

LbaCas12a (Cpf1) Nuclease recognizes T-rich TTTN PAM sequences, opening up additional genomic regions for gene targeting. The required gRNA is short, with only 40-44 bases. It has two nuclear localization signals, improving the efficiency of transportation to the nucleus. The cutting product is a 5 ‘protruding end. The activity temperature range of this enzyme is between 16 ℃ and 48 ℃. Compared with the homologous enzyme of Acidaminococcus, it is still active at lower temperatures and can be used to edit the genome of cold-blooded animals, such as Zebrafish and African clawed frog. High concentration enzymes can be used for micro injection, electroporation, and liposome transfection.

Component

Storage condition

Store at -30 ~ -15℃. Transportation conditions: ≤ 0 ℃.

Unit Definition

1 unit refers to the amount of Cas12a enzyme required to cleave 1 pmol ssDNA probe within 1 minute under 37 ℃ reaction conditions.

Source

Obtained through E. coli recombination, expression, and purification, the expressed gene is derived from Lachnospiraceae bacterium.

Applications

In vitro screening of efficient gRNA sequences, specific double stranded DNA cleavage guided by gRNA, selective linearization of double stranded DNA containing specific sequences, etc.

Description

CRISPR/Cas9 is an adaptive immune defense system formed by bacteria and archaea during their long-term evolution. The CRISPR/Cas9 system integrates fragments of invading phage and plasmid DNA into the CRISPR sequence and utilizes corresponding CRISPR RNAs (crRNAs) to guide the degradation of homologous sequences by Cas9 proteins, thereby providing immunity. The artificially modified Cas9/sgRNA system uses sgRNA (short guide RNA) to guide Cas9 proteins to recognize and cleave double stranded DNA with sgRNA targets, which can be used for gene knockout and precise DNA editing operations. The Cas9 nucleic acid Endonuclease provided by this product can enable the protein to enter the nucleus for Genome editing by adding a nuclear localization signal (NLS) to the N-terminal of the protein. This product is a pure protein system to avoid plasmid or virus interference during CRISPR gene knockout.

Component

Storage condition

Store at -30 ~ -15 ℃. Transportation conditions: ≤ 0 ℃.

Unit Definition

1 unit refers to the amount of enzyme required to add 0.5 pmol of dNTP to the acid insoluble precipitate during a 10 minute reaction at 30 ℃.

Source

Obtained through E. coli recombination, expression, and purification, the expressed gene is derived from Streptococcus pyogenes.

Applications

Cell gene editing, in vitro screening of efficient gRNA sequences, specific double stranded DNA cleavage guided by gRNA, selective linearization of double stranded DNA containing specific sequences, etc.

Description

Cas9 Nuclease is an RNA directed sequence specific double stranded DNA Endonuclease. Wizard RNA identifies target sites by complementing with the target sequence and carries Cas9 Nuclease onto the target DNA. Cas9 Nuclease has two cleavage active centers, which respectively cleave two strands of target DNA to produce DNA DNA repair Double-strand breaks. The cleavage site is located within the target region and three bases away from NGG (PAM).

Component

Storage condition

Store at -15~-30 ℃. Transportation conditions: ≤ 0 ℃.

Unit Definition

1 unit refers to the amount of enzyme required to add 0.5 pmol of dNTP to the acid insoluble precipitate during a 10 minute reaction at 30 ℃.

Source

This product is a high-purity Cas9 active protein expressed and purified in Escherichia coli using the Cas9 gene cloned from Streptococcus pyogenes.

Application

Double stranded DNA cleavage; Genomic modification.