Product Number: MR016

Shipping and Storage

Store at -20℃ with RNase Free Water as the storage buffer.

Components

Description

Recombinant human erythropoietin (EPO) is a glycoprotein hormone with a molecular weight of 34 kDa, composed of 165 amino acids, and is the main regulatory factor for mammalian regulation of red blood cell generation. Research has shown that transfection of EPO mRNA in vivo leads to a significant increase in reticulocyte and hematocrit measurements. This product effectively reduces the immunogenicity of mRNA expression in mammalian cells and enhances the stability of mRNA by introducing N1-methyl pseudouridine to replace the original UTP. It also simulates mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, making it an ideal choice for studying transfection and expression using various assays.

This product is a mature mRNA with a 5’Cap 1 structure and a 3’poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit (E131) and modified with Cap 1 Capping System Kit (M082). The sequence length is 1048nt.

Features

1. The Cap 1 structure is more suitable for mammalian systems and has higher translation efficiency than the Cap 0 structure (ARCA and m7Cap). Replacing UTP with modified base N1-Me Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving the efficiency of translation initiation.
2. The experimental method is simple and fast, with stable results and good reproducibility.
3. The mRNA transfection efficiency is stable.

Application

1. Gene substitution is a popular application of mRNA. The intracellular expression of mRNA greatly expands the number of possible indications for protein replacement therapy.
2. Human erythropoietin (hEPO) protein is a secreted protein that can be easily measured in serum through enzyme-linked immunosorbent assay. HEPO can stimulate the generation of red blood cells, which can be measured using hematocrit assay. HEPO mRNA can be used to mimic secreted proteins.

Quality control

There is no residual RNA enzyme, and mRNA purity, capping rate, and endotoxin testing are qualified.

Product Description

Luciferase is a general term for the enzymes that can produce biofluorescence in nature, the most representative of which is from the North American fluorescent worm (Photinus pyralis). The sequence of the firefly Luciferase mRNA (N1 Me Pseudo UTP) is from Photinus pyralis, and point mutation is carried out on the basis of the wild type sequence, significantly improving the stability of the protein and the suitable pH range. Once the product enters the cell, it will express Luciferase, Luciferase can catalyze the oxidation of the substrate D-luciferin to oxyluciferin. In the process of D-luciferin oxidation, bioluminescence will be generated at about 560nm wavelength and measured by a chemiluminescence meter or liquid scintillation meter. Firefly Luciferase is a commonly used bioluminescence reporter gene, which can be used as a control to study the translation efficiency of target genes in mammalian cells, Cell viability and in vivo imaging measurements. This product effectively reduces the autoimmunogenicity of mRNA in mammalian cells and enhances its stability by introducing N1-Me Pseudo UTP as a replacement for natural UTP. It also simulates mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, making it an ideal choice for studying transfection and expression using various assays

This product is a mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit and modified by the Cap 1 Capping System

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap) and has higher translation efficiency. Replacing UTP with modified base N1-Me-Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. mRNA is directly expressed in the cytoplasm and transfection efficiency is stable

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, and in vivo imaging

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency

Storage Condition

Store at -20 ℃ with RNase Free Water as the storage buffer

Packing Size

Product Description

EGFP mRNA (N1 Me Pseudo UTP) encodes a green fluorescent protein, whose maximum excitation/emission light wavelengths are 488nm/509nm respectively. Transfected cells can express enhanced green fluorescence, which can be used as a control to study the transfection and expression of target genes in mammalian cells. This product can effectively reduce the autoimmunity of mRNA in mammalian cells by introducing N1 Me Pseudo UTP to replace natural UTP, Enhance the stability of mRNA while also simulating mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, making it an ideal choice for studying transfection and expression using various assays

This product is a mature mRNA with a 5 ‘Cap 1 structure and a 3’ poly (A) tail, synthesized using the T7 High Yield RNA Transcription Kit and modified by the Cap 1 Capping System

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap) and has higher translation efficiency. Replacing UTP with modified base N1-Me-Pseudo UTP can reduce the intrinsic immune stimulation of IVT mRNA and enhance protein translation. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. mRNA is directly expressed in the cytoplasm and transfection efficiency is stable

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, and in vivo imaging

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency

Storage Condition

Store at -20 ℃

Product packaging

Product Description

Luciferase is a general term for the enzymes that can produce biofluorescence in nature, among which the most representative is the Luciferase from the body of the North American firefly, Luciferase. The firefly can display Luciferase activity without post translation modification

The sequence of this product is from the North American firefly (Photinus pyralis), and point mutation is carried out on the basis of the wild type sequence, which significantly improves the thermal stability and suitable pH value range of the protein. This product is synthesized by using the T7 High Yield RNA Transcription Kit, and Cap 1 Capping System is modified. It is a mature mRNA with 5 ‘Cap 1 structure and 3’ poly (A) tail, and the introduction of Cap1 structure and poly (A) tail structure, It increases the stability and translation efficiency of Luciferase mRNA, making it an ideal tool for studying transfection and protein translation

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap), with higher transcription efficiency. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. High transfection efficiency

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, etc

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band

Storage Condition

Store at -20 ℃

Packing Size

Note

The translation and expression of mRNA in vivo are directly related to the amount of mRNA added

Product Description

eGFP mRNA encodes a green fluorescent protein and can be expressed in mammalian cells. It simulates mature mRNA with a 5’Cap 1 structure and a 3’poly (A) tail, making it an ideal choice for studying transfection and expression using various assays.

This product is a mature mRNA with a 5’Cap 1 structure and a 3’poly (A) tail, synthesized using the T7 High Yield RNA Transcription kit and modified by the Cap 1 Capping System.

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap), with higher transcription efficiency. The addition of poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving the efficiency of translation initiation.

2. The experimental method is simple and fast, with stable results and good reproducibility.

3. High transfection efficiency.

Product Application

1. As a reporting material for gene regulation and functional research.

2. Suitable for detection of mRNA transmission, translation efficiency, cell viability, and in vivo imaging.

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band, and stable transfection efficiency.

Storage Condition

Store at -20 ℃.

Packing Size