Manual

Product Number: EK0939

Shipping and Storage

-20±5℃. For frequent use, store at 2-8℃ and avoid repeated freeze-thaw cycles.

Components

Description

SuperFastStar Universal Probe Mixture (UNG) is a 2× qPCR premix suitable for probe-based fluorescence quantitative detection. It contains Taq DNA Polymerase, Uracil-N-Glycosylase, PCR Buffer, dNTPs, Mg2⁺, K⁺, enhancers, and stabilizers. The Taq DNA Polymerase is a double-antibody-captured hot-start enzyme with polymerase activity inhibition rates exceeding 95% at 55℃ or below, effectively reducing nonspecific amplification at low temperatures. The added Uracil-N-Glycosylase and dUTP contamination prevention system catalyzes dsDNA and ssDNA containing uracil, releasing free uracil to minimize cross-contamination of amplification products. The unique PCR buffer system significantly enhances qPCR amplification efficiency, enabling accurate detection across a dynamic range of up to 6 logarithmic levels.

This product is suitable for single and multiple amplifications, featuring high detection sensitivity and excellent specificity. It can detect templates as low as single copies, supporting direct amplification of oral swabs and low-concentration blood samples. With strong versatility, it is widely used in gene expression and virus detection. Additionally, the premixed solution contains extremely low glycerol content, allowing direct freeze-drying with lyophilization protectants.