Manual

Product Number: SAP01

Shipping and Storage

Store at -20℃. Shelf‑life: 2 years.

Components

Description

Shrimp Alkaline Phosphatase (Shrimp Alkaline Phosphatase, rSAP) catalyzes the dephosphorylation of 5′ and 3′ phosphate monoesters on DNA and RNA. This phosphatase is widely used in molecular biology research, such as removing phosphate groups from the termini of DNA and RNA for subsequent cloning and probe end labeling. In cloning, it can dephosphorylate linear vectors to prevent self-ligation. Shrimp alkaline phosphatase is completely and irreversibly inactivated by incubation at 65°C for 5 minutes, eliminating the need to remove heat-sensitive phosphatases after ligation or end labeling. Based on these properties, it serves as an excellent alternative to the traditional phosphatase—Calf Intestinal Phosphatase (CIP).

Application

1. Dephosphorylation of DNA and RNA
2. Preventing the self-ligation of cloning vectors
3. Prepare the 5´-end labeled template

Unit Definition

1 unit refers to the amount of enzyme required to hydrolyze 1 umol of p-Nitrophenyl Phosphate (pNPP) to pNP per minute at 37℃.