Manual

Product Number: RE05883

Shipping and Storage

-20℃.

Components

Description

Nt.BstNBI is a type of endonuclease that cleaves only one strand of dsDNA substrate; Create incisions on the dsDNA substrate without incising the dsDNA.

Suggested reaction conditions

1. 10×Cut Buffer C.
2. Incubate at 55℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.
4. This product has 100% activity when subjected to enzymatic digestion at 37℃.
5. This product has 25% activity in FlashCut™ reaction buffer.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Definition of Activity

1 active unit (U) refers to the amount of enzyme required to completely convert 1µg of supercoiled pUC19 DNA into an open-loop form within 1 hour at 55℃ in a 50μL reaction system.

Quality control

1. Ultra long incubation test: incubate 10 U Nt. BstNBI with super spiral pUC19 DNA substrate at 55 ℃ for 16 h, and detect no change in open loop DNA by agarose gel electrophoresis.
2. RNase residue detection: incubate 10 U Nt. BstNBI and 500ng RNA at 37℃ for 1 h, and use agarose gel electrophoresis to detect that more than 90% of RNA remains intact.