Manual

Product Number: RE05880

Shipping and Storage

-20℃.

Components

Description

Nb.BsrDI is a type of endonuclease that cleaves only one strand of dsDNA substrate; Create incisions on the dsDNA substrate without incising the dsDNA.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer.
2. Incubate at 65℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.
4. This product has 50% activity when subjected to enzymatic digestion at 37℃.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Definition of Activity

1 active unit (U) refers to the amount of enzyme required to completely convert 1µg of supercoiled pUC19 DNA into an open-loop form within 1 hour at 65℃ in a 50μL reaction system.

Quality control

1. Ultra long incubation test: incubate 10 U Nb. BsrDI and super spiral pUC19 DNA substrate at 65℃ for 16 h, and detect no change in open loop DNA by agarose gel electrophoresis.
2. RNase residue detection: incubate 10 U Nb. BsrDI and 500ng RNA at 37℃ for 1 h, and use agarose gel electrophoresis to detect that more than 90% of RNA remains intact.