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User Manual

ELK17J1280, Mouse Eukaryotic Translation Initiation Factor 5 (EIF5)

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Mouse EIF5 ELISA Kit instruction
Intended use
This EIF5 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of EIF5 in the sample, this EIF5 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus EIF5 concentration. The concentration of EIF5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storages
Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃or -80℃.Avoid repeated freeze-thaw cycles.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.
Materials required but not supplied

1. Standard microplate reader(450nm).
2. Precision pipettes and Disposable pipette tips.
3. 37 ℃incubator.
   Precautions

   Name	96 determinations	48 determinations
   Microelisa stripplate	8\\*12strips	8\\*6strips
   Standard	0.3ml\\*6tubes	0.3ml\\*6tubes
   Sample Diluent	6.0ml	3.0ml
   HRP-Conjugate reagent	10.0ml	5.0ml
   20X Wash solution	25ml	15ml
   Chromogen Solution A	6.0ml	3.0ml
   Chromogen Solution B	6.0ml	3.0ml
   Stop Solution	6.0ml	3.0ml
   Closure plate membrane	2	2
   User manual	1	1
   Storage: 2-8℃. Validity:six months.
   FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!