Manual

Product Number: RA01

Shipping and Storage

Sealed, dry.

Description

RNase A is an endonuclease that has been well studied and widely used. RNase A hydrolyzes RNA.

Application

1. Drugs: change host cell metabolism, inhibit virus synthesis,
2. Biochemical experiments: Catalyzed RNA degradation, which can specifically hydrolyze the RNA phosphate diester bond hybridized to THE DNA chain, so it can decompose the RNA chain in the RNA/DNA hybrid system.
3. Industrial production，decompose RNA and purify the product.

Specification

1. Source: Bovine (Porcine) pancreas.
2. Appearance: White or pale yellow lyophilized powder.
3. Solvent Transparentness: Not more opalescent than ref.suspensionⅡ.
4. Loss on drying: No more than 5.0%.
5. DNA: Not detectable.
6. Microbial limits: Pseudomonas aeruginosa: Negative; Salmonella: Negative; Staphylococcus aureus: Negative
7. Assay: No less than 50Kunitz units/mg
8. Protein (UV): No less than 65%

Order

Specifications and Analysis

The following parameters represent the standard quality profile for this product based on the Certificate of Analysis:

Storage and Stability

Retest Interval: 24 months from the date of production when stored under recommended conditions.
Handling: Keep container tightly closed in a dry and well-ventilated place.
Safety: Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.

Product Number: RPC001

Description

Type I collagen is a vital component of human skin. Its fibrous bundle structure provides the skin with strong support and toughness, helping to maintain firmness and elasticity. This Recombinant Humanized Type I Collagen is derived from microorganisms. It is characterized by low impurity and high purity, which effectively reduces the risk of allergic reactions. Utilizing advanced protein engineering technology and a 28,000-liter yeast platform, the product ensures high purity and activity while remaining free of bacterial endotoxins.

Features

• High Purity & Safety: Features high purity and activity with no bacterial endotoxins.
• Low Allergy Risk: Microbe-derived with low impurities to minimize allergic risks.
• Moisturizing: Excellent hydrophilicity and moisture retention for deep skin hydration.
• Cost-Effective: Advanced production platforms reduce costs, providing a cost-effective raw material for beauty and medical device R&D.

Application

Suitable for the face, eye area, neck, and other regions:

• Improves the skin barrier and enhances immune function.
• Inhibits tyrosinase to reduce melanin deposition.
• Locks in moisture to prevent dry lines and improves the skin microbiome.

Quality Control

• Grade: Molecular biology grade, sterile lyophilized.
• CAS No.: 9064-67-9.
• Molecular Weight: 26.9 KD.
• Source: Microorganism (Human gene sequence).
• Purity (SDS-PAGE): > 90%.
• Protein Content (Lowry): > 90%.
• Water Content: < 10%.
• Endotoxin: < 0.5 EU/mg.
• Microbial Limits: Total bacterial count < 100 Cfu/g; Total fungal count < 10 Cfu/g.
• Residual Heavy Metals: < 10 ppm (as Pb).

Storage

Valid for at least 1 year when stored in a dry environment below -20°C after receipt.

Specification

Shipping and Storage

Ice pack transportation; Stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Component

Description

Exonuclease I is derived from the recombinant expression of the Exo I gene in E. coli strains. This enzyme has the activity of hydrolyzing single stranded DNA from the 3-5 “direction, gradually releasing deoxyribonucleic acid 5” monophosphate and leaving intact 5 “terminal dinucleotides. This product is mainly used for the degradation and digestion of primers after PCR amplification, and is inactive for double stranded DNA and 3OH terminal DNA chains blocked by phosphoryl or acetyl groups.

Application

Commonly used to remove single stranded primers in PCR reactions before Sanger sequencing or SNP analysis; Remove single stranded primers from nested PCR; Purification of PCR products by enzymatic method; Remove single stranded DNA containing 3 ‘hydroxyl terminus from nucleic acid mixtures.

Specification

1. Source: Escherichia coli strain carrying the exonuclease I gene cloned from E. coli NM554.
2. Enzyme storage solution: 10mM Tris HCl, 100mM NaCl, 5mM β – ME, 0.5mM EDTA, 100µg/ml BSA, 50% Glycerol pH 7.5 @ 25℃.
3. Unit definition: 1 unit refers to the amount of enzyme required to catalyze the release of 10nmol of acid soluble nucleosides within 30 minutes at 37℃.
4. Inactivation or inhibition: react at 80℃ for 20 minutes.

Product Number: S120308

Shipping and Storage

Wet ice transportation; -Stored at 20℃, with a validity period of 12 months.

Component

Description

Lambda Exonuclease, also known as lambda Exonuclease or lambda exonuclease, is a 5 ‘-3’ deoxyribonuclease derived from the exonuclease gene of lambda bacteriophages and recombinant expressed in Escherichia coli. It can specifically hydrolyze the 5 ‘end phosphorylated strand in double stranded DNA along the 5’ -3 ‘direction, but has low enzymatic activity for single stranded DNA and non phosphorylated dsDNA at the 5’ end, and cannot be digested from DNA cuts or gaps.

Application

Production of single stranded PCR products, analysis of DNA single stranded conformational polymorphism, rolling ring amplification, PCR product cloning, etc.

Features

1. Source: Derived from λ bacteriophage, recombinant expressed by Escherichia coli.
2. Enzyme activity definition: The amount of enzyme required to hydrolyze 10nmol of acid soluble deoxyribonucleotide from a double stranded DNA substrate in a 50μL reaction system at 37℃ within 1 minute is defined as one enzyme activity unit.
3. Purity and concentration: SDS-PAGE detection shows a purity of ≥ 95%; Endogenous nucleic acid residue<1 pg/μL (qPCR detection); Enzyme activity is 5U/μL.
4. Inactivation or inhibition: Incubate at 75℃ for 10 minutes to inactivate.
5. Enzyme storage buffer: 25mM Tris HCl, 50mM NaCl, 1 mM DTT, 0.1mM EDTA, 50% Glycerol, pH 8.
6. 10×Reaction Buffer: 670mM Glycine-KOH, 25mM MgCl₂, 500μg/mL BSA, pH 9.4.

Product Number: DA43S

Shipping and Storage

Store at 2 ~ 8°C in the dark, transport with ice packs; recommend Equalbit dsDNA BR Standard 2 for long-term storage at -30 ~ -15°C.

Component

Description

The Equalbit dsDNA BR (Broad Range) Assay Kit is a simple, sensitive, and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit includes fluorescent detection reagents, buffer, and dsDNA standards. It exhibits excellent linearity for dsDNA samples in the range of 4–2,000ng, enabling precise quantification of samples with concentrations between 0.2–2,000ng/μL. The kit demonstrates higher selectivity for dsDNA compared to RNA and maintains good tolerance to common contaminants such as salts, free nucleotides, proteins, solvents, and detergents. This product is easy to operate and can be performed at room temperature. Before use, dilute the fluorescent detection reagent with buffer to prepare the working solution, then add the dsDNA sample to be tested. The Qubit fluorescence instrument can then be used for detection.

Scope of Application

dsDNA samples with concentrations ranging from 0.2 to 2,000ng/μL.

Product Number: PSE109

Shipping and Storage

1. Storage temperature: -20±5℃ for storage
2. Validity period: 2 years.

Component

Description

N-glycosidase F (PNGase F) is an effective amidase produced by recombinant Escherichia coli with cloned genes encoding the enzyme. It can cleave the N-sugar chain on glycoproteins, helping to generate carbohydrate free peptides and oligosaccharides with di-N-acetyl chitosan units. N-glycosidase F is the most effective method for removing almost all N-sugar chains from glycoproteins. The product can remove all complex, heterozygous, and high mannose sugar chains from antibodies and their fusion proteins, but if there are alpha 1,3-linked fucose residues in the core structure (often expressed in plant and insect cells as immunoglobulins), they cannot be cleaved. Obtaining accurate N-sugar chain distribution in the shortest possible time is crucial for effective process control. N-glycosidase F is an optimized reagent that can rapidly deglycosylate antibodies and fusion proteins within a few minutes. All N-sugar chains can be released quickly and without preference, and can be directly subjected to downstream chromatography or mass spectrometry analysis to quickly determine the glycosylation of antibodies.

Active definition

Unit enzyme activity is defined as the amount of enzyme required to remove over 95% of sugar chains from 10μg denatured RNase B within 1 hour in a 10μL reaction system at 37℃.

Product Number: PCK34

Shipping and Storage

Stored at -20℃, valid for 12 months.

Component

Description

This kit is specifically designed for one-step RT-PCR experiments, and can be used to conveniently and quickly complete reverse transcription and PCR amplification reactions in the same reaction tube. During the reaction process, there is no need to open the tube cap to add reagents, which avoids contamination while improving detection sensitivity and experimental efficiency. This kit includes the OneStep Enzyme Mix optimized for optimal ratio (including mutated TRUEscript M-MuLV H Minus reverse transcriptase, hot start HotMaster Taq DNA polymerase, and RNasin inhibitor mix), as well as a unique 2 × RT-PCR Mix reaction system suitable for reverse transcription and PCR amplification. The RNase H activity of this enzyme M-MuLV (RNase H ˉ) is deficient. Compared with M-MuLV, it has stronger extensibility and stability, and can be used for longer cDNA synthesis and the construction of high proportion full-length cDNA libraries. At the same time, the enzyme enhances heat resistance and can reverse transcribe at 42-50 ° C, improving the efficiency of complex secondary structures and GC rich template reverse transcription.

Application

Suitable for high copy and low copy gene testing; RNA templates with high GC content or complex secondary structures.

Product Number: EK0504

Shipping and Storage

Room temperature (15-30℃).

Component

Description

RNA molecular diagnosis is a branch of molecular diagnosis in in vitro diagnostic technology (IVD). Compared with DNA molecular diagnosis, RNA molecular diagnosis has the advantages of high specificity, sensitivity, accuracy, speed, pollution-free, and good clinical conformity. In recent years, with the advancement of technology, RNA molecular diagnosis technology has attracted much attention.

When collecting blood samples, the gene expression profile can undergo significant changes within a few minutes. Anticoagulants such as EDTA can prevent blood clotting, but cannot prevent RNA in the blood from being rapidly degraded by RNAses. Therefore, when studying gene expression using blood samples, it is necessary to extract RNA from nucleated cells in fresh blood in a timely manner.

This product can stably store RNA in blood at room temperature (15-30℃) for at least 7 days without degradation, and at 4℃ for 14 days. Collecting blood samples is simple and convenient, and can be widely used in hospitals, research institutes, and third-party testing institutions for the collection and preservation of blood samples.

Features

1. Easy to save. Blood samples can be collected directly at room temperature to protect the RNA in the sample from degradation.
2. Negative pressure sampling. Vacuum treatment is applied to the storage tube, and the entire sampling process does not come into contact with blood samples, ensuring safety and speed.
3. Extraction is simple. The blood stored in the preservation tube can be matched with various downstream RNA extraction methods, such as Trizol method, centrifugal column method, magnetic bead automated extraction, etc.

Product Number: PCM18

Shipping and Storage

For long-term storage, please store in the dark at -20℃. After thawing, it can be stably stored for one month at 4℃ in the dark, avoiding repeated freezing and thawing as much as possible.

Component

Description

Universal Visual SYBR qPCR Master Mix is a specialized qPCR reagent for SYBR Green I chimeric dye method. It is a 2 × premix containing all qPCR components except primers and DNA samples, which can reduce operation steps, shorten sample addition time, and lower the risk of contamination. Its core component is antibody modified hot start Taq DNA polymerase, combined with carefully optimized buffer system and PCR reaction promoter, which has strong specificity and high amplification efficiency, and can effectively inhibit non-specific amplification. It can accurately quantify templates with a wide concentration range and obtain stable and reliable qPCR results. This product already contains universal calibration dyes and is compatible with the vast majority of qPCR devices, without the need for additional dyes to calibrate the instrument.

This product can utilize the color changing effect generated by mixing different dyes to track the pipetting process, thereby significantly reducing pipetting errors. The Universal Visual SYBR qPCR Master Mix contains blue dye, while the 10 × Dilution Buffer contains yellow dye. When a template diluted with Dilution Buffer (yellow) is added to the Universal Visual SYBR qPCR Master Mix (blue), a color change effect from blue to green occurs, allowing for accurate determination of whether the template has been added based on the liquid color.